1. How do viruses manipulate stem cells?
What is the link between HPC subsets and cellular regulation with viral latency?
How does an infrequent HCMV infection of a minor subset of HPCs result in global effects in the bone marrow and on immune responses?
2. What are the mechanisms of viral pathogenesis for beta-herpesviruses?
How do the HCMV RL11 genes contribute to latency & reactivation?
Which cellular pathways are controlling (or controlled by) the HCMV lifecycle?
3. How can we build better bone marrow?
How can we expand HPCs in vitro and maintain their function?
How can we specifically control HPC differentiation to replenish immune function?
How can we leverage the strength of beta-herpesvirus specificity to understand normal stem cell function?